HMC3-CAS9 cell line

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HMC3-CAS9 Order

Catalog#

YC-C131-Cas9-H

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Datasheet

Order

Detailed Product Information

Product category

Cas9 Stable Cell Lines

cell name

HMC3-CAS9

Cell Morphology

Epithelial-like, adherent

Passage ratio

1：2~1：3

Species

Human

Expression gene

Cas9

Resistance gene

hygro

Construction method

Lentivirus

Mycoplasma test

negative

Culture method

90%MEM+10%FBS

Description

Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the HMC3 cell line， screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a HMC3-CAS9 cell line that can maintain long-term, stable and high expression.

Product Validation Data（RT-QPCR）

Sample Name	Target Name	Cт Mean	ΔCт
HMC3-CAS9	Cas9	21.16933632	2.45841599
HMC3-CAS9	β-actin	18.71092033	2.45841599
HMC3	Cas9	31.39811516	14.71577072
HMC3	β-actin	16.68234444	14.71577072

Cutting Efficiency Validation

HMC3-CAS9 cell line Product Validation Data（RT-QPCR）

Note: The above figure shows the sequencing peaks of the HMC3-CAS9 stable cell pool which is electroporated by CLEC3B gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:HMC3			STR Info (Cell bank) Cell Line:HMC3
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	11	12		11	12
D13S317	11	11		11	11
D7S820	9	11		9	11
D16S539	12	13		12	13
VWA	17	19		17	19
TH01	6	6		6	6
AMEL	X	X		X	X
TPOX	8	9		8	9
CSF1PO	10	11		10	11
D12S391	16	21
FGA	21	25
D2S1338	17	25
D21S11	30	31.2
D18S51	18	18
D8S1179	13	14
D3S1358	16	18
D6S1043	11	11
PENTAE	7	13
D19S433	15	15.2
PENTAD	10	14
D1S1656	12	13

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
01
High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
02
High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
03

HMC3-CAS9

Catalog#

YC-C131-Cas9-H

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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HMC3-CAS9 cell line

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